Chromatographic Methods in Metabolomics
The concept of a metabolic profile was introduced in 1971, when gas chromatography demonstrated a range of compounds present in human samples. Now termed metabolomics, the field is still emerging, and chromatography remains an essential tool for determining metabolites in a living system. This is the first book to present the chromatographic techniques used in metabolomics in a fundamental way. Sample preparation and quality control are described in detail, and all forms of chromatography applied to metabolomics are included. The editors present guidelines on selecting the most appropriate methodology, making the book an accessible guide to anyone entering the field. Handling data and applications are also described. This is an essential handbook for any laboratory looking to embark on a metabolomics research programme and includes the fundamentals of chromatography alongside the latest developments in the field.
Chromatographic Methods in Metabolomics, The Royal Society of Chemistry, 2013.
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CHAPTER 1: Selection of Analytical Methodology for Metabolomicsp1-10BySusanne K. Wiedmer;Susanne K. WiedmerDepartment of ChemistryUniversity of Helsinki, P.O. Box 55, 00014 University of HelsinkiFinlandSearch for other works by this author on:Tuulia HyötyläinenTuulia HyötyläinenVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:
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CHAPTER 2: Sample Collection, Storage and Preparationp11-42ByTuulia HyötyläinenTuulia HyötyläinenVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:
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CHAPTER 3: Mass Spectrometric Detection for Chromatographyp43-63ByTuulia HyötyläinenTuulia HyötyläinenSearch for other works by this author on:
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CHAPTER 4: Liquid Chromatographic Techniques in Metabolomicsp64-86ByGeorgios A. Theodoridis;Georgios A. TheodoridisDepartment of ChemistryAristotle University Thessaloniki, 541 24 ThessalonikiGreeceSearch for other works by this author on:Filippos Michopoulos;Filippos MichopoulosDepartment of ChemistryAristotle University Thessaloniki, 541 24 ThessalonikiGreeceOncology IM, AstraZenecaMereside, Alderley Park, Macclesfield, Cheshire SK10 4TGUKSearch for other works by this author on:Helen G. Gika;Helen G. GikaDepartment of Chemical EngineeringAristotle University Thessaloniki, 541 24 ThessalonikiGreeceSearch for other works by this author on:Robert S. Plumb;Robert S. PlumbDepartment of Surgery and OncologySir Alexander Fleming Building, Imperial College, Exhibition Road, South Kensington, London SW7 2AZUK[email protected]Search for other works by this author on:Ian D. WilsonIan D. WilsonDepartment of Surgery and OncologySir Alexander Fleming Building, Imperial College, Exhibition Road, South Kensington, London SW7 2AZUK[email protected]Search for other works by this author on:
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CHAPTER 5: Gas Chromatographic Techniques in Metabolomicsp87-113ByChristian J. Wachsmuth;Christian J. WachsmuthInstitute of Functional GenomicsUniversity of Regensburg, Josef‐Engert‐Strasse 9, 93053 RegensburgGermany[email protected]‐regensburg.deSearch for other works by this author on:Franziska C. Vogl;Franziska C. VoglInstitute of Functional GenomicsUniversity of Regensburg, Josef‐Engert‐Strasse 9, 93053 RegensburgGermany[email protected]‐regensburg.deSearch for other works by this author on:Peter J. Oefner;Peter J. OefnerInstitute of Functional GenomicsUniversity of Regensburg, Josef‐Engert‐Strasse 9, 93053 RegensburgGermany[email protected]‐regensburg.deSearch for other works by this author on:Katja DettmerKatja DettmerInstitute of Functional GenomicsUniversity of Regensburg, Josef‐Engert‐Strasse 9, 93053 RegensburgGermany[email protected]‐regensburg.deSearch for other works by this author on:
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CHAPTER 6: Capillary Electromigration Techniques in Metabolomicsp114-137ByAkiyoshi Hirayama;Akiyoshi HirayamaInstitute for Advanced BiosciencesKeio University, Tsuruoka, Yamagata 997‐0052Japan[email protected]Search for other works by this author on:Tomoyoshi SogaTomoyoshi SogaInstitute for Advanced BiosciencesKeio University, Tsuruoka, Yamagata 997‐0052Japan[email protected]Search for other works by this author on:
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CHAPTER 7: Microchip Technology in Metabolomicsp138-182ByTiina M. SikanenTiina M. SikanenUniversity of HelsinkiFaculty of Pharmacy, Division of Pharmaceutical Chemistry, Viikinkaari 5E, 00014 HelsinkiFinland[email protected]Search for other works by this author on:
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CHAPTER 8: Data Handlingp183-194ByTuulia Hyötyläinen;Tuulia HyötyläinenVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:Matej OrešičMatej OrešičVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:
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CHAPTER 9: Applicationsp195-232ByPäivi Pöhö;Päivi PöhöVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:Maarit Kivilompolo;Maarit KivilompoloVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:Monica Calderon‐Santiago;Monica Calderon‐SantiagoDepartment of Analytical ChemistryUniversity of Córdoba Agroalimentary Excellence Campus, Campus of Rabanales, 14071 CórdobaSpainSearch for other works by this author on:Sirkku Jäntti;Sirkku JänttiVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:Susanne K. Wiedmer;Susanne K. WiedmerVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:Tuulia HyötyläinenTuulia HyötyläinenVTT Technical Research Centre of FinlandTietotie 2, P.O. Box 1000, 02044 VTT, EspooFinland[email protected]Search for other works by this author on:
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