CHAPTER 13: NMR Spectroscopy of Cell Culture, Tissues, and Other Biofluids
-
Published:04 Jan 2018
-
Special Collection: 2018 ebook collectionSeries: New Developments in NMR
T. J. Athersuch, C. Lau, V. Behrends, and H. C. Keun, in NMR-based Metabolomics, ed. H. C. Keun, The Royal Society of Chemistry, 2018, pp. 324-359.
Download citation file:
NMR spectroscopy can provide a wealth of information on cellular metabolism and is frequently used in metabolomics application that use cultured cells, tissues, and whole organisms. Central to these analyses are the protocols for sample harvest, which incorporate procedures for quenching metabolic processes to preserve samples in a state that is representative of their source. In this chapter, the main considerations are discussed with reference to literature exemplars. In the latter half of the chapter, less commonly studied biofluids that also have specific sample preparation requirements are discussed, with a focus on cerebrospinal fluid, faeces, bile, seminal fluid, and milk.