CHAPTER 5: High-resolution Magic-angle Spinning (HR-MAS) NMR Spectroscopy
Published:04 Jan 2018
Special Collection: 2018 ebook collectionSeries: New Developments in NMR
A. Wong and C. Lucas-Torres, in NMR-based Metabolomics, ed. H. C. Keun, The Royal Society of Chemistry, 2018, pp. 133-150.
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Since the beginning of high-resolution magic-angle spinning (HR-MAS) NMR spectroscopy in 1990s, we have witnessed tremendous instrumentation and methodological advancements in the HR-MAS NMR technique for semisolids. With HR-MAS, it is now possible to acquire reliable high-quality spectra in a routine and high-throughput fashion, and it has become a well-integrated metabolic screening tool for ex vivo biospecimens such as tissue biopsies, cells and organisms for NMR-based metabolomics research. This chapter provides the basic principles of HR-MAS and describes a few recent noteworthy developments that could strengthen the role of HR-MAS as a frontline NMR technique for metabolomics.