Microalgal Hydrogen Production: Achievements and Perspectives
CHAPTER 5: Assessment of Electrochemically-based Strategies to Protect [FeFe]-hydrogenases from Oxygen
Published:19 Mar 2018
Special Collection: 2018 ebook collection , ECCC Environmental eBooks 1968-2022
E. Rogers, C. F. Megarity, J. Esselborn, M. Winkler, T. Happe, and F. A. Armstrong, in Microalgal Hydrogen Production: Achievements and Perspectives, ed. M. Seibert and G. Torzillo, The Royal Society of Chemistry, 2018, pp. 139-154.
Download citation file:
The concept of tethering an efficient four-electron oxidase to a photosynthetic [FeFe] hydrogenase to improve O2 tolerance during H2 production by green algae has been explored experimentally. Protein film electrochemistry has been used to determine how well a blue Cu oxidase that is co-adsorbed with hydrogenase on an electrode surface is able to act as a ‘bodyguard’ to offer protection against O2. The results show that protection is afforded in the potential region favouring H2 oxidation, but not in the potential region favouring H2 evolution—conditions under which the [FeFe]-hydrogenase is naturally more O2-resilient.