Microalgal Hydrogen Production: Achievements and Perspectives
CHAPTER 5: Assessment of Electrochemically-based Strategies to Protect [FeFe]-hydrogenases from Oxygen
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Published:19 Mar 2018
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Elliott Rogers, Clare F. Megarity, Julian Esselborn, Martin Winkler, Thomas Happe, Fraser A. Armstrong, 2018. "Assessment of Electrochemically-based Strategies to Protect [FeFe]-hydrogenases from Oxygen", Microalgal Hydrogen Production: Achievements and Perspectives, Michael Seibert, Giuseppe Torzillo
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The concept of tethering an efficient four-electron oxidase to a photosynthetic [FeFe] hydrogenase to improve O2 tolerance during H2 production by green algae has been explored experimentally. Protein film electrochemistry has been used to determine how well a blue Cu oxidase that is co-adsorbed with hydrogenase on an electrode surface is able to act as a ‘bodyguard’ to offer protection against O2. The results show that protection is afforded in the potential region favouring H2 oxidation, but not in the potential region favouring H2 evolution—conditions under which the [FeFe]-hydrogenase is naturally more O2-resilient.
