Microalgal Hydrogen Production: Achievements and Perspectives
CHAPTER 6: Sustaining Hydrogen Production in Eukaryotic Microalgae Through Genetic Approaches
Published:19 Mar 2018
Special Collection: 2018 ebook collection , ECCC Environmental eBooks 1968-2022
Olaf Kruse, Deepak Venkanna, 2018. "Sustaining Hydrogen Production in Eukaryotic Microalgae Through Genetic Approaches", Microalgal Hydrogen Production: Achievements and Perspectives, Michael Seibert, Giuseppe Torzillo
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The efficiency of H2 production with microalgae is generally hampered by the fact that under in vivo conditions the bio-catalytic conversion of protons and electrons to hydrogen is only used as a limited valve system, reacting to severe stress conditions to prevent cell damage caused by an imbalance of the cellular redox-homeostasis. The participating hydrogenase enzyme in eukaryotic microalgae has a large enzymatic capacity; however, high H2-production rates are mainly prevented by insufficient delivery of protons and electrons. To overcome this bottleneck, molecular engineering of specific parts of the cellular metabolism has been considered as a promising approach. Recent achievements using forward and reverse genetic approaches for the identification and construction of efficient, eukaryotic-microalgal H2 producers resulted in the isolation of high H2 producers with production rates up to 850 ml H2 l−1 cell culture. Application strategies include systematic genomics to identify and isolate new, high H2, GMO-cell lines through different screening programs from libraries constructed by random mutagenesis, as well as targeted mutagenesis approaches combined with systems biology required for the localization of potential bottlenecks.