Microalgal Hydrogen Production: Achievements and Perspectives
CHAPTER 7: Metabolism and Genetics of Algal Hydrogen Production
Published:19 Mar 2018
Special Collection: 2018 ebook collection , ECCC Environmental eBooks 1968-2022
Matthew C. Posewitz, 2018. "Metabolism and Genetics of Algal Hydrogen Production", Microalgal Hydrogen Production: Achievements and Perspectives, Michael Seibert, Giuseppe Torzillo
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Algal hydrogenase research continues to provide important new insights into the origin and function of H2 metabolism in photoautotrophic microorganisms. Additionally, powerful new genetic tools are emerging to manipulate algae, which when combined with the discovery of promising new enzymes are enabling advanced recombinant techniques with the potential to improve H2 production in photoautotrophic organisms. In recent years, more O2-tolerant enzymes have been reported, and “clean” backgrounds that have endogenous hydrogenase activity eliminated in the model organism Chlamydomonas reinhardtii have emerged. Recombinant enzymes are now readily expressed and tested in this system and initial results show promise for improved H2 production. Several algal genomes/transcriptomes have also recently become available, providing important insights into the evolution and function of native hydrogenases in algae. These efforts suggest an evolutionary relationship among algal hydrogenases that implies a single, ancient incorporation of H2 metabolism in eukaryotic algae. In summary, the algal hydrogenase field has advanced dramatically in recent years and additional important insights are likely in the coming years.