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The exploration of hydrogels as biomaterials requires evaluation of the biocompatibility of the hydrogelators because the cytotoxicity or the biocompatibility of the hydrogelators will ultimately decide the applications of the hydrogels. In this chapter, we focus on the examination of the hydrogelators based on small peptide derivatives. Using several representative hydrogelators of small peptide derivatives, we first introduce studies that explore the biocompatibility of these hydrogelators in mammalian cells, and then summarize several works on the biocompatibility of the hydrogelators in animal models. By illustrating the biocompatibility of various types of hydrogelators containing small peptides, we hope this chapter will provide the basic framework for the design of hydrogelators of small peptide derivatives for biomedical applications.

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