Chapter 4: Scanning Electrochemical Microscopy (SECM): Fundamentals and Applications in Life Sciences

In recent years, scanning electrochemical microscopy (SECM) has made significant contributions to the life sciences. Innovative developments focusing on high-resolution imaging, developing novel operation modes, and combining SECM with complementary optical or scanning probe techniques renders SECM an attractive analytical approach. This chapter gives an introduction to the essential instrumentation and operation principles of SECM for studying biologically-relevant systems. Particular emphasis is given to applications aimed at imaging the activity of biochemical constituents such as enzymes, antibodies, and DNA, which play a pivotal role in biomedical diagnostics. Furthermore, the unique advantages of SECM and combined techniques for studying live cells is highlighted by discussion of selected examples.