CHAPTER 4: Matrix Biology: Structure and Assembly of Laminin-Rich Matrices
Published:18 Nov 2015
K. J. Hamill, S. B. Hopkinson, N. M. E. Hill, and J. C. R. Jones, in Mimicking the Extracellular Matrix: The Intersection of Matrix Biology and Biomaterials, ed. G. A. Hudalla and W. L. Murphy, The Royal Society of Chemistry, 2015, pp. 103-158.
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In this review we focus on one of the most diverse families of matrix molecules, namely the laminins. Their diversity and the fact that their expression is tightly regulated during development and tissue remodeling would indicate that they are central to tissue organization. We will detail laminin structure, expression profiles, and outline the diseases which are a result of their aberrant expression and/or function. We will also describe laminin network formation, signaling regulated by laminin–cell-surface receptor interactions, and modification of laminin functionality through laminin-binding proteins. Understanding the biology of the laminins, how they assemble, and the nature of their binding partners is crucial, since laminins are widely used as substrates for the culture of cells in vitro and are being developed for use in bioengineering and clinical applications in vivo. Hence, we will provide information on the types, functions and interactions of laminins currently available from commercial and academic sources or whose recombinant production has been described. Finally, we will discuss the potential use of laminins in tissue engineering, focusing on their utility to enhance material cellularization.