Protein and Peptide Analysis by LC-MS: Experimental Strategies
This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.
Protein and Peptide Analysis by LC-MS: Experimental Strategies, The Royal Society of Chemistry, 2011.
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Top Down and Bottom Up Analysis of Proteins (Focusing on Quantitative Aspects)p1-10ByFriedrich LottspeichFriedrich LottspeichMax Planck Institute of BiochemistryProtein Analysis, Am Klopferspitz 18, 82152 MartinsriedGermanySearch for other works by this author on:
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Chapter 2: How to Couple and Handle Liquid Chromatography with Mass Spectrometryp11-25ByThomas LetzelThomas LetzelCompetence Pool Weihenstephan (CPW)Center of Life and Food Sciences WeihenstephanTechnische Universität München, Weihenstephaner Steig 2385350 FreisingGermanySearch for other works by this author on:
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Chapter 3: Expression and Purification of Bioactive Proteins/Peptides with Conventional Liquid Chromatographyp26-37ByTakayuki Ohnuma;Takayuki OhnumaKinki UniversityDepartment of Advanced Bioscience, 3327–204 NakamachiNara631–8505JapanSearch for other works by this author on:Tamo FukamizoTamo FukamizoKinki UniversityDepartment of Advanced Bioscience, 3327–204 NakamachiNara631–8505JapanSearch for other works by this author on:
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Chapter 4: Liquid Chromatography-Mass Spectrometry of Intact Proteinsp38-55ByNicolas L. Young;Nicolas L. YoungPrinceton UniversityDepartment of Molecular Biology, PrincetonNJ 08544USASearch for other works by this author on:Benjamin A. GarciaBenjamin A. GarciaPrinceton UniversityDepartment of Molecular Biology, PrincetonNJ 08544USAPrinceton UniversityDepartment of Chemistry, PrincetonNJ 08544USASearch for other works by this author on:
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Chapter 5: LC-MS(/MS) of Trypsin-Hydrolysed Proteinsp56-70BySeronei C. Cheison;Seronei C. CheisonZentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL): Bioactive Peptides and Protein TechnologyTechnische Universität München, Weihenstephaner Berg 1, D-85354 FreisingGermanySchool of Public Health and Community DevelopmentMaseno University, Private Bag, KisumuKenyaSearch for other works by this author on:Ulrich M. KulozikUlrich M. KulozikZentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL) Abteilung Technologie, Lehrstul für Lebensmittelverfahrenstechnik und MolkereitechnologieTechnische Universität München, Weihenstephaner Berg 1, D-85354 FreisingGermanySearch for other works by this author on:
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Chapter 6: On-line Protein Digestion in Combination with Chromatographic Separation and Mass Spectrometric Detectionp71-86ByS. Johannes Hoos;S. Johannes HoosVU UniversityFaculty of SciencesBioMolecular Analysis Group, De Boelelaan 1083, 1081 HVAmsterdamThe NetherlandsSearch for other works by this author on:Wilfried M.A. NiessenWilfried M.A. NiessenVU UniversityFaculty of SciencesBioMolecular Analysis Group, De Boelelaan 1083, 1081 HVAmsterdamThe NetherlandsSearch for other works by this author on:
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Chapter 7: Bioinformatic Tools for the LC-MS/MS Analysis of Proteins and Peptidesp87-103ByChristian Webhofer;Christian WebhoferMax Planck Institute of PsychiatryProteomics and Biomarkers, Professor Dr. Christoph W. Turck, Kraepelinstrasse 2-1080804 MunichGermanySearch for other works by this author on:Michael SchraderMichael SchraderWeihenstephan-Triesdorf University of Applied SciencesDepartment of Biotechnology and Bioinformatics, 85350 FreisingGermanySearch for other works by this author on:
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Chapter 8: Quantitative LC-MS of Proteinsp104-122ByGabriele Stöhr;Gabriele StöhrMax Planck Institute of BiochemistryDepartment of Proteomics and Signal Transduction, Am Klopferspitz 18, 82152 MartinsriedGermanySearch for other works by this author on:Andreas TebbeAndreas TebbeKINAXO BiotechnologiesAm Klopferspitz 19a, 82152 MartinsriedGermanySearch for other works by this author on:
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Chapter 9: LC-MS for the Identification of Post-Translational Modifications of Proteinsp123-132ByBoris MacekBoris MacekProteome Center TübingenInterdepartmental Institute for Cell BiologyUniversity of Tübingen, Auf der Morgenstelle 1572076 TübingenGermanySearch for other works by this author on:
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Chapter 10: LC-MS for the Determination of the Enzymatic Activity of Proteinsp133-141ByRomy K. Scheerle;Romy K. ScheerleInstitute for Chemical-Technical Analysis and Chemical Food TechnologyCenter of Life and Food Sciences Weihenstephan, Technische Universität München, Weihenstephaner Steig 23, 85350 FreisingGermanySearch for other works by this author on:Johanna GraßmannJohanna GraßmannInstitute for Chemical-Technical Analysis and Chemical Food TechnologyCenter of Life and Food Sciences Weihenstephan, Technische Universität München, Weihenstephaner Steig 23, 85350 FreisingGermanySearch for other works by this author on:
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Chapter 11: Functional Analysis of Proteins, Including LC-MS and Special Freewarep142-155ByMichael Krappmann;Michael KrappmannInstitut für Forschung und WeiterbildungUniversity of Applied Sciences, Hochschule Weihenstephan – TriesdorfAm Hofgarten 485350 FreisingGermanySearch for other works by this author on:Thomas LetzelThomas LetzelCompetence Pool Weihenstephan (CPW)Technische Universität München, Weihenstephaner Steig 2385350 FreisingGermanySearch for other works by this author on:
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Chapter 12: Industrial Standards and Strategies in LC-MS Analysis of Proteinsp156-167ByRene WissiackRene WissiackBiotech OperationsProcess Science, In-Process-Control, Boehringer Ingelheim RCV GmbH & Co KG, Dr. Boehringer-Gasse 5-11, 1121 WienAustriaSearch for other works by this author on:
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