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RSC Chromatography Monographs
Protein and Peptide Analysis by LC-MS: Experimental Strategies
Edited by
Thomas Letzel
Thomas Letzel
Technische Universität München, Germany
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Hardback ISBN:
978-1-84973-182-9
PDF ISBN:
978-1-84973-314-4
Special Collection:
2011 ebook collection
,
2011 ebook collection
,
2011-2015 analytical chemistry subject collection
No. of Pages:
194
Publication date:
22 Jul 2011
Book Chapter
Chapter 9: LC-MS for the Identification of Post-Translational Modifications of Proteins
By
Boris Macek
Boris Macek
Proteome Center Tübingen
Interdepartmental Institute for Cell Biology
University of Tübingen, Auf der Morgenstelle 15
72076 Tübingen
Germany
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-
Published:22 Jul 2011
-
Special Collection: 2011 ebook collection , 2011 ebook collection , 2011-2015 analytical chemistry subject collection
Page range:
123 - 132
Citation
B. Macek, in Protein and Peptide Analysis by LC-MS: Experimental Strategies, ed. T. Letzel, The Royal Society of Chemistry, 2011, ch. 9, pp. 123-132.
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The chemical and physical diversity of post-translational modifications (PTMs) of proteins presents a challenge in determining their structure and function. Liquid chromatography–mass spectrometry (LC-MS) is currently the method of choice for the analysis of protein modifications, but a proper workflow has to be developed and applied to each modification studied. This chapter presents major challenges in the identification of PTMs by LC-MS and discusses a protocol for large-scale enrichment and LC-MS analysis of protein phosphorylation.
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