Chapter 12: Industrial Standards and Strategies in LC-MS Analysis of Proteins

Mass spectrometry in process control of recombinant protein production plays an important role in industry due to its versatility. If a generic chromatographicdesalting method, column switching technique or direct infusion electrospray mass spectrometry is used in conjunction with it, reliable results are obtained within a short period of time. The measurement results can be directly related to theoretically calculated values; consequently, information about the status of theprotein under investigation is givendirectly. The analysis of protein solutions by means of HPLC-MS can bestraightforwardlyautomated,and short overall analysis times are feasible. Finally,the high degree of flexibility of this technique assists urgent problem-solving in routineapplications.