Unravelling Single Cell Genomics
Chapter 8: Looking at the DNA of a Single Cell
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Published:18 Oct 2010
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Special Collection: 2010 ebook collection , 2010 ebook collection , 2010 materials and nanoscience subject collectionSeries: Nanoscience & Nanotechnology
B. Polzer and C. A. Klein, in Unravelling Single Cell Genomics, ed. N. Bontoux, M. Potier, L. Dauphinot, H. Craighead, H. Kroto, and P. O'Brien, The Royal Society of Chemistry, 2010, ch. 8, pp. 73-80.
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Small amounts of genomic DNA frequently impede genetic analyses in many fields of research, including forensic research, prenatal diagnosis or, as discussed earlier in chapter 6, oncology. To overcome these limitations amplification of genomic DNA may provide quantities of DNA needed for downstream applications. This chapter will describe methods currently available to analyse the DNA of single cells. A focus will be on whole genome amplification (WGA) of single cells and the advantages and drawbacks of available protocols will be discussed.
