Unravelling Single Cell Genomics
Chapter 8: Looking at the DNA of a Single Cell
Published:18 Oct 2010
Special Collection: 2010 ebook collection , 2010 ebook collection , 2010 materials and nanoscience subject collectionSeries: Nanoscience & Nanotechnology
Bernhard Polzer, Christoph A. Klein, 2010. "Looking at the DNA of a Single Cell", Unravelling Single Cell Genomics, Jean-Christophe Baret, Bruno Cauli, Max Chabert, Valerie Abecassis-Taly, Petra Dittrich, Emmanuel Fort, Christoph Klein, Joel Lachuer, Bertrand Lambolez, Nicholas Le Novere, Severine le Gac, Laili Mahmoudian, Yann Marcy, Bernhard Polzer, Joelle Vinh, Tania Vitalis, Nathalie Bontoux, Marie-Claude Potier, Luce Dauphinot, Harold Craighead, Harry Kroto, Paul O'Brien, Royal Society of Chemistry
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Small amounts of genomic DNA frequently impede genetic analyses in many fields of research, including forensic research, prenatal diagnosis or, as discussed earlier in chapter 6, oncology. To overcome these limitations amplification of genomic DNA may provide quantities of DNA needed for downstream applications. This chapter will describe methods currently available to analyse the DNA of single cells. A focus will be on whole genome amplification (WGA) of single cells and the advantages and drawbacks of available protocols will be discussed.