Unravelling Single Cell Genomics
Chapter 12: Cell Capture and Lysis on a Chip
Published:18 Oct 2010
Special Collection: 2010 ebook collection , 2010 ebook collection , 2010 materials and nanoscience subject collectionSeries: Nanoscience & Nanotechnology
Séverine Le Gac, Albert van den Berg, 2010. "Cell Capture and Lysis on a Chip", Unravelling Single Cell Genomics, Jean-Christophe Baret, Bruno Cauli, Max Chabert, Valerie Abecassis-Taly, Petra Dittrich, Emmanuel Fort, Christoph Klein, Joel Lachuer, Bertrand Lambolez, Nicholas Le Novere, Severine le Gac, Laili Mahmoudian, Yann Marcy, Bernhard Polzer, Joelle Vinh, Tania Vitalis, Nathalie Bontoux, Marie-Claude Potier, Luce Dauphinot, Harold Craighead, Harry Kroto, Paul O'Brien, Royal Society of Chemistry
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Single cell analysis in a microfluidic device proceeds through a certain number of steps. The two first steps of the protocol are the manipulation of individual cells in the microfluidic device and their positioning in precise locations and subsequently the process to rupture the cell membrane to retrieve the cellular content. In this chapter, we will focus on these two first steps of cell trapping and single cell lysis, and review different approaches and techniques found in the literature for these two steps to achieve these in a microfluidic format. We will also discuss the advantages and limitations of the different techniques of manipulation, trapping and lysis.