Unravelling Single Cell Genomics
Chapter 17: Tiny Droplets for High-throughput Cell-based Assays
Published:18 Oct 2010
Special Collection: 2010 ebook collection , 2010 ebook collection , 2010 materials and nanoscience subject collectionSeries: Nanoscience & Nanotechnology
J.-C. Baret, V. Taly, 2010. "Tiny Droplets for High-throughput Cell-based Assays", Unravelling Single Cell Genomics, Jean-Christophe Baret, Bruno Cauli, Max Chabert, Valerie Abecassis-Taly, Petra Dittrich, Emmanuel Fort, Christoph Klein, Joel Lachuer, Bertrand Lambolez, Nicholas Le Novere, Severine le Gac, Laili Mahmoudian, Yann Marcy, Bernhard Polzer, Joelle Vinh, Tania Vitalis, Nathalie Bontoux, Marie-Claude Potier, Luce Dauphinot, Harold Craighead, Harry Kroto, Paul O'Brien, Royal Society of Chemistry
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In order to perform a high number of assays on biological objects or chemical compounds which are sometimes impossible to obtain in large quantities, miniaturizing experiments has been the main issue. Standard technologies, such as microtiter plates have nearly reached the smallest sizes of samples they can handle. In order to miniaturize the assays, a further decrease in the size of the reactors in which reactions are performed is required. The use of small droplets provides new ways to miniaturize assays; moreover droplets are ideal for compartmentalizing biological objects or compounds. Droplets of equal sizes are produced in series and manipulated on demand providing quantitative and miniaturized versions of the microtiter plate assays and enables the parallelization of the assay.
In this chapter we will review droplet-based microfluidics: droplet production, flow, fusion, sorting, and detection. We will discuss how to design reactions in droplets and particularly how to maintain living cells in droplets and run biological reactions.