Unravelling Single Cell Genomics
Chapter 11: Microfluidics: Basic Concepts and Microchip Fabrication
Published:18 Oct 2010
Special Collection: 2010 ebook collection , 2010 ebook collection , 2010 materials and nanoscience subject collectionSeries: Nanoscience & Nanotechnology
Conni Vollrath, Petra S. Dittrich, 2010. "Microfluidics: Basic Concepts and Microchip Fabrication", Unravelling Single Cell Genomics, Jean-Christophe Baret, Bruno Cauli, Max Chabert, Valerie Abecassis-Taly, Petra Dittrich, Emmanuel Fort, Christoph Klein, Joel Lachuer, Bertrand Lambolez, Nicholas Le Novere, Severine le Gac, Laili Mahmoudian, Yann Marcy, Bernhard Polzer, Joelle Vinh, Tania Vitalis, Nathalie Bontoux, Marie-Claude Potier, Luce Dauphinot, Harold Craighead, Harry Kroto, Paul O'Brien, Royal Society of Chemistry
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Microfluidic devices are nowadays versatile platforms for analytical applications and chemical syntheses, and are frequently used for cell culturing and cell studies. What are the unique features of a microfluidic device and how do these properties support the development of novel analytical methods for investigations on the single cell level? This chapter introduces to the basic concepts and aspects of microfluidics. It gives newcomers an understanding of the special characteristics such as the laminar flow and segmented flow. Furthermore, a short overview of fabrication methods is given highlighting the most popular approaches, particularly soft lithography. Finally, various materials useful for microchip fabrication are described and discussed with a special emphasis on their applicability for cell analytical devices.