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Two-dimensional (2D) materials have drawn considerable attention in the past decade owing to their physicochemical properties and its broad range applications in various fields such as energy storage and generation, opto-electronic devices and chemical sensors. The present chapter gives an overview on the experimental approaches of 2D materials and its different forms as a key constituent in the nanocomposite preparation and its utilization as electrode material in the fabrication of modified electrodes in sensing of diverse analyte molecules of special interest. In particular, the potential applications of 2D materials in photoelectrochemical sensing, electrogenerated chemiluminescence sensing, optical and electrochemical sensing followed by wearable sensors are also summarized. Some of the latest discoveries pertaining to the real sample applications are also discussed in detail.

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