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Microbial natural products has drawn a great attention from both academia and industry. Obtaining the planar, relative, and possibly the absolute configuration of such novel metabolites requires considerable efforts. In this chapter, the general NMR experiments that were traditionally used, and the recently introduced pulse sequences that provide more precise output in significantly shorter times, and applicable to high throughput screening are discussed. Additionally, various dereplication approaches that allow tracking of new metabolites were highlighted. Lastly, the power of CASE in the structural identification and confirmation of manually-elucidated molecules using selected examples from our research projects was demonstrated.

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