Singlet Oxygen: Applications in Biosciences and Nanosciences
Chapter 13: Genetically Encoded Singlet Oxygen Photosensitizers
Published:27 Jan 2016
Special Collection: 2016 ebook collection
R. Ruiz-González, A. Rodríguez-Pulido, J. Torra, S. Nonell, and C. Flors, in Singlet Oxygen: Applications in Biosciences and Nanosciences, ed. S. Nonell, C. Flors, S. Nonell, and C. Flors, The Royal Society of Chemistry, 2016, ch. 13, pp. 271-286.
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The ability to selectively control the site of singlet-oxygen (1O2) production in a cell is crucial for inflicting photodynamic damage in a desired location. Genetically encoded photosensitizers, which can be fused to virtually any protein and are expressed in a cell without the need to add any external cofactors, offer absolute control of the 1O2 production site. This chapter provides a molecular view of the photosensitization mechanisms in two families of genetically encoded photosensitizers: green fluorescent protein variants and flavoproteins. It also covers the main applications of these proteins in areas such as photodynamic therapy and chromophore-assisted laser inactivation, as well as in advanced imaging techniques.