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The present contribution provides an overview of the latest research studies in which Nitrones and Nitroxides are involved in the applications of Electron Paramagnetic Resonance Spectroscopy (EPR) in different fields. Due to the free radical nature of Nitroxides (Aminoxyls), their use in EPR investigations is rather straightforward, while for Nitrones (N-oxides) it relies instead on their use as “radical scavengers”, and/or by their use in the EPR Spin Trapping technique. Since the chemistry of both Nitrones and Nitroxides have been extensively described elsewhere, this contribution would only represent an update on some of their most recent application involving EPR spectroscopy.

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