Chapter 10: Cell-like liposomes integrated with microfluidic technology for synthetic biology Check Access
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Published:02 Jun 2014
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T. Osaki, K. Kamiya, and S. Takeuchi, in Synthetic Biology, Volume 1, ed. M. Ryadnov, L. Brunsveld, and H. Suga, The Royal Society of Chemistry, 2014, vol. 1, ch. 10, pp. 275-291.
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Microfluidic devices have substantially advanced the field of synthetic biology with the generation of model cells, thus achieving (1) the efficient encapsulation of cytosolic materials that enabled cell-free protein expression, (2) the incorporation of membrane proteins that allowed the regulation of membrane permeability, (3) the programmable control of the model-cell size, and (4) the high throughput generation of the model cells. In this chapter, we introduce the cutting edge of the microfluidic technologies customized for the production of cell-like liposomes.
