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Chemical sensors and assays are essential to determine the concentration or functional activity of (bio)analytes. Most established assays rely on biomolecular recognition such as antibody–antigen interactions or protein–ligand interactions. However, during the past decade, supramolecular receptors have proven as useful alternatives affording new assays for hitherto undetectable analytes as well as for the development of refined assay procedures. Herein, we review sensors and assays, in which cucurbiturils (CBs) are key components. We focus on optical methods such as absorption, fluorescence, and circular dichroism spectroscopy, and we also include a summary of NMR-based bioimaging with CBs.

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