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Drug Discovery
Biomedical Imaging: The Chemistry of Labels, Probes and Contrast Agents
Edited by
Martin Braddock
Martin Braddock
AstraZeneca, UK
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Hardback ISBN:
978-1-84973-014-3
PDF ISBN:
978-1-84973-291-8
Special Collection:
2011 ebook collection
,
2011 ebook collection
,
2011-2015 analytical chemistry subject collection
Series:
Drug Discovery
No. of Pages:
552
Published online:
23 Nov 2011
Published in print:
30 Nov 2011
Book Chapter
Chapter 7.2: Fluorescence Lifetime Imaging applied to Microviscosity Mapping and Fluorescence Modification Studies in Cells
By
Klaus Suhling
;
Klaus Suhling
Department of Physics
King's College London, Strand, London WC2R 2LS
UK
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Nicholas I. Cade
;
Nicholas I. Cade
Department of Physics
King's College London, Strand, London WC2R 2LS
UK
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James A. Levitt
;
James A. Levitt
Department of Physics
King's College London, Strand, London WC2R 2LS
UK
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Marina K. Kuimova
;
Marina K. Kuimova
Department of Chemistry
Imperial College London, Exhibition Road, London SW7 2AZ
UK
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Pei-Hua Chung
;
Pei-Hua Chung
Department of Physics
King's College London, Strand, London WC2R 2LS
UK
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Gokhan Yahioglu
;
Gokhan Yahioglu
Department of Chemistry
Imperial College London, Exhibition Road, London SW7 2AZ
UK
PhotoBiotics Ltd
21 Wilson Street, London EC2M 2TD
UK
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Gilbert Fruhwirth
;
Gilbert Fruhwirth
Randall Division of Cell and Molecular Biophysics and Division of Cancer Studies, Guy's Medical School Campus
King's College London, London SE1 1UL
UK
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Tony Ng
;
Tony Ng
Randall Division of Cell and Molecular Biophysics and Division of Cancer Studies, Guy's Medical School Campus
King's College London, London SE1 1UL
UK
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David Richards
David Richards
Department of Physics
King's College London, Strand, London WC2R 2LS
UK
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Published:23 Nov 2011
-
Special Collection: 2011 ebook collection , 2011 ebook collection , 2011-2015 analytical chemistry subject collectionSeries: Drug Discovery
Page range:
371 - 390
Citation
K. Suhling, N. I. Cade, J. A. Levitt, M. K. Kuimova, P. Chung, G. Yahioglu, ... D. Richards, in Biomedical Imaging: The Chemistry of Labels, Probes and Contrast Agents, ed. M. Braddock, The Royal Society of Chemistry, 2011, ch. 7.2, pp. 371-390.
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Fluorescence lifetime imaging (FLIM) is a key optical technique for imaging the photophysical environment of fluorescent probes in vivo. In addition, it also provides information in fluorescence enhancement studies that intensity imaging alone could not provide. We review the principles and recent advances in the application of the techniques, instrumentation and molecular probe development.
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