Chapter 4: Isotopic Techniques Combined with ICP-MS and ESI-MS
Published:03 Jun 2010
M. Wang, W. Feng, and Z. Chai, in Nuclear Analytical Techniques for Metallomics and Metalloproteomics, ed. C. Chen, Z. Chai, and Y. Gao, The Royal Society of Chemistry, 2010, ch. 4, pp. 95-127.
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Isotopic techniques combined with ICP-MS and ESI-MS have been successfully applied for study of proteomics and metallomics in recent years. This chapter is divided in six sections and introduces these techniques and applications. In the first two sections, the principle and instrumentation of ICP-MS and ESI-MS are summarized. The two powerful techniques are really complementary for metallomics and metalloproteomics study, because ICP-MS has a unique quantitative ability and an unmatched sensitivity for elemental detection, whereas ESI-MS can provide information on protein sequence. In the following three sections, commonly used isotopic techniques combined with ICP-MS and ESI-MS are introduced respectively, including isotopic tracer techniques, isotope dilution analysis and isotope labeling techniques for protein quantification. The unique characteristics and successful applications of these isotopic techniques are highlighted. Finally, it is concluded that isotopic techniques combined with ICP-MS and ESI-MS are very promising for metallomics and metalloproteomics and more successful advances are expected in future.