Miniaturization and Mass SpectrometryCheck Access
About this book
The recent explosion in the use of analytical chemistry, particularly in the biological sciences, has led to a need for fast, reliable and highly sensitive tools able to handle small sample sizes. This book illustrates how microfluidics and lab-on-a-chip devices can satisfy the growing need for miniaturized and enhanced analysis. They lend themselves well to mass spectrometric detection as they use samples in the low microlitre range and are handled on a chip. Miniaturization and Mass Spectrometry focuses on one particular technique, mass spectrometry, whose popularity has increased dramatically in the last two decades with the increase in use of biological analysis and the development of two "soft" ionization techniques, ESI and MALDI. These enable the analysis of large but fragile biological molecules such as DNA, proteins and oligosaccharides. The book starts with an introduction to the coupling of microfluidics to mass spectrometry techniques. It then goes on demonstrate the advantages of such a coupling: the MS analysis benefits from improved sample preparation when performed on a chip while MS yields more information on the sample handled on the chip compared to conventional optical detection. A history on the developments in this field, starting from the off-chip coupling to the on-chip ionization, is also provided. Daniel Figeys, a pioneer in the development of microfluidic systems for MS analysis, describes the early beginnings of this hyphenated analysis technique. Solutions to couple microfluidic systems to the two most popular ionization methods, ESI and MALDI, are presented throughout the chapters. Various examples are given of the application of this microfluidics-MS hyphenated analysis technique to proteomics, metabolomics, organic chemistry and forensics. Coverage is not limited to academic research. The development of commercialized systems and their current use for routine biological analysis are also presented. Lastly, a future vision of the integration of the mass spectrometer on the chip is raised, as a last step to yield fully portable systems for on-site analysis.
Miniaturization and Mass Spectrometry, The Royal Society of Chemistry, 2008.
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Table of contents
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Chapter 1: Introductionp1-20BySéverine Le Gac;Séverine Le GacBIOS the Lab-on-a-Chip Group, University of TwentePO Box 2177500 AE EnschedeThe NetherlandsSearch for other works by this author on:Albert van den BergAlbert van den BergBIOS the Lab-on-a-Chip Group, University of TwentePO Box 2177500 AE EnschedeThe NetherlandsSearch for other works by this author on:
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Chapter 2: The Coupling of Microfabricated Fluidic Devices with Electrospray Ionization Mass Spectrometersp25-43ByDaniel Figeys;Daniel FigeysOttawa Institute of Systems Biology, University of Ottawa451 Smyth RoadOttawaOntarioCanada K1H 8M5Search for other works by this author on:Ruedi aebersoldRuedi aebersoldInstitute of Molecular Systems Biology, ETH Zürich, and Faculty of Science, University of Zürich, Switzerland, and Institute for Systems BiologySeattleWA 98103USASearch for other works by this author on:
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Chapter 3: A Silicon-based ESI Chip with Integrated Counter Electrode and its Applications Combined with Mass Spectrometryp47-66ByGary A. SchultzGary A. SchultzAdvion BioSciences, Inc.Advion BioSystems19 Brown RoadIthacaNY 14850USASearch for other works by this author on:
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Chapter 4: Microfabricated Multichannel Electrospray Ionization Emitters on Polydimethylsiloxane (PDMS) Microfluidic Devicesp67-96ByJin-Sung Kim;Jin-Sung KimMedical University of South CarolinaCharlestonSC 29425USASearch for other works by this author on:Daniel R. KnappDaniel R. KnappMedical University of South CarolinaCharlestonSC 29425USASearch for other works by this author on:
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Chapter 5: Microfabricated Nanoelectrospray Emitter Tips based on a Microfluidic Capillary Slotp97-128BySéverine Le Gac;Séverine Le GacUniversité des Sciences et Technologies de Lille (Lille 1), UFR de ChimieBâtiment C4UMR CNRS 8009Chimie Organique et Macromoléculaire59655 Villeneuve d’Ascq CedexFranceSearch for other works by this author on:Steve Arscott;Steve ArscottInstitut d’Electronique, de Microélectronique et de Nanotechnologies (IEMN), UMR CNRS 8520Avenue PoincaréCité ScientifiqueVilleneuve d’Ascq CedexFranceSearch for other works by this author on:Christian RolandoChristian RolandoUniversité des Sciences et Technologies de Lille (Lille 1), UFR de ChimieBâtiment C4UMR CNRS 8009Chimie Organique et Macromoléculaire59655 Villeneuve d’Ascq CedexFranceSearch for other works by this author on:
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Chapter 6: Microfabricated Parylene Electrospray Tips Integrated with Cyclo-Olefin Microchips for ESI-MSp129-148ByYanou Yang;Yanou YangBristol Myers Squibb Research and DevelopmentPenningtonNJ 08534USASearch for other works by this author on:Jack D. Henion;Jack D. HenionAdvion BiosciencesIthacaNY 14850USASearch for other works by this author on:H. G. CraigheadH. G. CraigheadApplied and Engineering Physics, Cornell UniversityIthacaNY 14853USASearch for other works by this author on:
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Chapter 7: Microfluidic Bioanalytical Platforms with Mass Spectrometry Detection for Biomarker Discovery and Screeningp151-172ByIulia M. LazarIulia M. LazarVirginia Bioinformatics Institute and Department of Biological Sciences, Virginia Polytechnic Institute and State UniversityWashington St. VBI/Bio IIRoom 283BlacksburgVA 24061USASearch for other works by this author on:
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Chapter 8: Modular Microfluidics Devices Combining Multidimensional Separations: Applications to Targeted Proteomics Analyses of Complex Cellular Extractsp173-197ByMihaela Ghitun;Mihaela GhitunDepartment of Chemistry, Université de MontréalPO Box 6128Station Centre-villeMontréalCanadaH3C 3J7Institute for Research in Immunology and Cancer, Université de MontréalPO Box 6128Station Centre-villeMontréalCanadaH3C 3J7Search for other works by this author on:Eric Bonneil;Eric BonneilInstitute for Research in Immunology and Cancer, Université de MontréalPO Box 6128Station Centre-villeMontréalCanadaH3C 3J7Search for other works by this author on:Christelle Pomiès;Christelle PomièsInstitute for Research in Immunology and Cancer, Université de MontréalPO Box 6128Station Centre-villeMontréalCanadaH3C 3J7Search for other works by this author on:Maria Marcantonio;Maria MarcantonioInstitute for Research in Immunology and Cancer, Université de MontréalPO Box 6128Station Centre-villeMontréalCanadaH3C 3J7Search for other works by this author on:Hongfeng Yin;Hongfeng YinAgilent Technologies5301 Stevens Creek BlvdSanta ClaraCA 95051USASearch for other works by this author on:Kevin Killeen;Kevin KilleenAgilent Technologies5301 Stevens Creek BlvdSanta ClaraCA 95051USASearch for other works by this author on:Pierre ThibaultPierre ThibaultDepartment of Chemistry, Université de MontréalPO Box 6128Station Centre-villeMontréalCanadaH3C 3J7Institute for Research in Immunology and Cancer, Université de MontréalPO Box 6128Station Centre-villeMontréalCanadaH3C 3J7Search for other works by this author on:
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Chapter 9: Simple Chip-based Interfaces for On-line Nanospray Mass Spectrometryp201-236ByMonica Brivio;Monica BrivioLaboratory of Supramolecular Chemistry and Technology, University of TwentePO Box 2177500 AE EnschedeThe NetherlandsSearch for other works by this author on:Willem Verboom;Willem VerboomLaboratory of Supramolecular Chemistry and Technology, University of TwentePO Box 2177500 AE EnschedeThe NetherlandsSearch for other works by this author on:David N. ReinhoudtDavid N. ReinhoudtLaboratory of Supramolecular Chemistry and Technology, University of TwentePO Box 2177500 AE EnschedeThe NetherlandsSearch for other works by this author on:
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Chapter 10: On-line and Off-line MALDI from a Microfluidic Devicep239-255ByHarrison K. Musyimi;Harrison K. MusyimiDepartment of Chemistry and Center for BioModular Multi-Scale Systems, Louisiana State UniversityBaton RougeLA 70803USASearch for other works by this author on:Steven A. Soper;Steven A. SoperDepartment of Chemistry and Center for BioModular Multi-Scale Systems, Louisiana State UniversityBaton RougeLA 70803USASearch for other works by this author on:Kermit K. MurrayKermit K. MurrayDepartment of Chemistry and Center for BioModular Multi-Scale Systems, Louisiana State UniversityBaton RougeLA 70803USASearch for other works by this author on:
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Chapter 11: Lab-on-a-Chip Devices Enabling (Bio)chemical Reactions with On-line Analysis by MALDI-TOF Mass Spectrometryp256-276ByMonica Brivio;Monica BrivioLaboratory of Supramolecular Chemistry and Technology, University of TwentePO Box 2177500 AE EnschedeThe NetherlandsSearch for other works by this author on:Willem Verboom;Willem VerboomLaboratory of Supramolecular Chemistry and Technology, University of TwentePO Box 2177500 AE EnschedeThe NetherlandsSearch for other works by this author on:David N. ReinhoudtDavid N. ReinhoudtLaboratory of Supramolecular Chemistry and Technology, University of TwentePO Box 2177500 AE EnschedeThe NetherlandsSearch for other works by this author on:
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Chapter 12: MALDI-TOF Mass Spectrometry and Digital Microfluidics for the Investigation of Pre-steady State Enzyme Kineticsp277-288ByKevin P. Nichols;Kevin P. NicholsMESA+Institute for Nanotechnology, Mesoscale Chemical Systems, University of TwentePostbus 217, 7500 AE EnschedeThe NetherlandsSearch for other works by this author on:Han J. G. E. GardeniersHan J. G. E. GardeniersMESA+Institute for Nanotechnology, Mesoscale Chemical Systems, University of TwentePostbus 217, 7500 AE EnschedeThe NetherlandsSearch for other works by this author on:
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Chapter 13: Development of Miniaturized MALDI Time-of-Flight Mass Spectrometers for Homeland Security and Clinical Diagnosticsp291-310ByRobert J. Cotter;Robert J. CotterJohns Hopkins University School of MedicineDepartment of Pharmacology & Molecular SciencesMiddle Atlantic Mass Spectrometry LaboratoryBaltimoreMD 21205USASearch for other works by this author on:Sara McGrath;Sara McGrathJohns Hopkins University School of MedicineDepartment of Pharmacology & Molecular SciencesMiddle Atlantic Mass Spectrometry LaboratoryBaltimoreMD 21205USASearch for other works by this author on:Christine Jelinek;Christine JelinekJohns Hopkins University School of MedicineDepartment of Pharmacology & Molecular SciencesMiddle Atlantic Mass Spectrometry LaboratoryBaltimoreMD 21205USASearch for other works by this author on:Theresa Evans-NguyenTheresa Evans-NguyenJohns Hopkins University School of MedicineDepartment of Pharmacology & Molecular SciencesMiddle Atlantic Mass Spectrometry LaboratoryBaltimoreMD 21205USASearch for other works by this author on:
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