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A photoimmobilization technology using photoreactive and nonbiofouling polymers was developed for the preparation of microarray biochips. This photoimmobilization enabled the convenient covalent immobilization of various types of biological components. In addition, by using nonbiofouling polymers as matrixes it was possible to reduce the nonspecific interactions with the biological components. Various proteins, allergens, autoantigens, viruses, and cells were microarrayed using this technique. This microarray technology will be useful not only for fundamental investigations of biological interactions, but also for applications in clinical analysis.

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