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In vitro cell culture experiments that determine cell adhesion, proliferation and differentiation are valuable tools for evaluating the biocompatibility of biomaterial modifications with charged surfaces. This Chapter will give a short overview of the basic principles of cell adhesion, then discuss some of the practical aspects of determining the proliferation of adherent cells and review cell differentiation with a focus on the osteogenic path. Finally, the impact of charged surfaces on the described cell behaviour is discussed.

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