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The extreme sensitivity of chemiluminescence analysis in combination of the high specificity of antibodies has resulted in many application of chemiluminescence immunoassays (CLIA) over the last three decades. Especially the reproducible analysis in complex matrices leads to the success of this method in clinical, food and environmental analysis. Principles of CLIAs will be addressed as well current improvements of CL labelling, CLIA processing and instrumentation. Many applications for CL-ELISA, CL-Microarrays, Flow-injection CLIA are given. Commercialised singleplex CLIA platforms as well new multiplexed CLIA platforms are presented and discussed in detail.

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