Handbook of Culture Media for Food and Water Microbiology
Bile Aesculin Agar (BAA)
Published:06 Dec 2011
Special Collection: 2011 ebook collection , 2011 ebook collection , 2011-2015 food science subject collection
2011. "Bile Aesculin Agar (BAA)", Handbook of Culture Media for Food and Water Microbiology, Janet E L Corry, Gordon D W Curtis, R M Baird
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This is a Provisional monograph.
In 1924, Rochaix used the characteristic of aesculin hydrolysis in the identification of enterococci; later, Meyer and Schonfield (1926) incorporated bile into the medium as a selective agent for their isolation. Swan (1954) used 40% bile salt and aesculin hydrolysis to demonstrate that isolation of characteristic colonies on the medium correlated with Lancefield Group D antigen. In practice, this medium is recommended as an alternative to kanamycin aesculin azide agar for the rapid confirmation of enterococci isolated on M-Enterococcus agar (Anon., 2006).
Dissolve the ingredients in the water and adjust the solution to a pH value of 7.1±0.2. Sterilise at 121°C for 15 min. The final pH should be 7.1±0.2.