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This monograph has been reviewed by members of the IUMS–ICFMH Working Party on Culture Media and given ‘Proposed’ status.

This medium consists of peptone, sodium chloride and yeast extract as basal ingredients, with the cefoperazone amphotericin teicoplanin (CAT) supplement of Atabay and Corry (1998). Atabay et al. (2002) used it for the isolation of low numbers of Arcobacter spp. from poultry and red meat in conjunction with a non-selective blood agar and after membrane filtration.

Suspend the above ingredients except for the cefoperazone sodium salt, amphotericin B and teicoplanin in 900 mL of water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 min. Cool to 50°C. Suspend the antibiotics in 100 mL of sterile water and add to the 900 mL of basal broth, mixing gently. The broth can be dispensed in universal bottles or tubes. Nutrient broth no. 2 may be substituted for the basal ingredients and the antibiotic mixture may be obtained commercially.

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