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This is a Provisional monograph.

M-PA was formulated by Levin and Cabelli in 1972. The formulation has been modified by raising the pH (Carson et al., 1975; M-PA-B) and further modified by Brodsky and Ciebin (1978), who eliminated sulfapyridine and cyclohexamide and re-named the medium M-PA-C. M-PA-C is less inhibitory and enables the isolation and enumeration of Pseudomonas aeruginosa in 24 h at 41.5°C. It is described in the American standard method for Pseudomonas aeruginosa (APHA, 2005) as modified M-PA agar.

Dissolve the ingredients except the antibiotics in boiling water. Cool to 55°C, adjust the pH to 7.2 and add 1 mL of a 0.8% filter-sterilised solution of nalidixic acid and 1 mL of a 3.7% filter-sterilised solution of kanamycin per 1000 mL of medium.

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