Handbook of Culture Media for Food and Water Microbiology
Chapter G1: Giolitti and Cantoni broth with Tween 80 (GCBT)
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Published:06 Dec 2011
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Special Collection: 2011 ebook collection , 2011 ebook collection , 2011-2015 food science subject collection
2011. "Giolitti and Cantoni broth with Tween 80 (GCBT)", Handbook of Culture Media for Food and Water Microbiology, Janet E L Corry, Gordon D W Curtis, R M Baird
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This monograph has been assessed by members of the IUMS–ICFMH Working Party on Culture Media and given ‘Approved’ status.
Giolitti and Cantoni broth was developed for the recovery of low numbers of coagulase-positive staphylococci, in particular Staphylococcus aureus, in foods (Giolitti and Cantoni, 1966). It contains sodium pyruvate to enhance repair of stressed staphylococci and to promote growth. Addition of Tween 80 is necessary for the successful recovery of Staphylococcus aureus (Chopin et al., 1985). Mannitol is used as the carbohydrate source whilst lithium chloride, potassium tellurite and glycine inhibit competitive microorganisms. Anaerobic growth conditions increase the selectivity of the medium.
