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This monograph has been reviewed by members of the IUMS–ICFMH Working Party on Culture Media and given ‘Proposed’ status.

Listeria monocytogenes blood agar (LMBA) was developed by Johansson and Kankare (1996) for the specific isolation of Listeria monocytogenes from food and environmental samples. LMBA uses trypticase soy agar as a base with lithium chloride, polymyxin B sulfate and ceftazidime as selective agents and 5% sheep blood as an indicator of haemolysis. LMBA is capable of distinguishing Listeria monocytogenes directly from other species by demonstration of β-haemolysis and, in contrast to some other selective plating media, does not show false-negative results due to overgrowth of other Listeria spp. after enrichment. Presumptive Listeria monocytogenes colonies are surrounded by a typical haemolytic zone and usually visible after an incubation period of 24–48 h.

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