Handbook of Culture Media for Food and Water Microbiology
Chapter G2: Glycine Vancomycin Polymyxin Cycloheximide (GVPC) agar
Published:06 Dec 2011
Special Collection: 2011 ebook collection , 2011 ebook collection , 2011-2015 food science subject collection
2011. "Glycine Vancomycin Polymyxin Cycloheximide (GVPC) agar", Handbook of Culture Media for Food and Water Microbiology, Janet E L Corry, Gordon D W Curtis, R M Baird
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This is a Provisional monograph.
Glycine vancomycin polymyxin cycloheximide agar is a selective medium for the detection of Legionella species and was developed by Dennis et al. in 1984 from the glycine vancomycin polymyxin agar of Wadowsky and Yee (1981). They added α-ketoglutarate and cycloheximide to suppress the growth of yeasts and fungi. Vancomycin and polymyxin B sulfate inhibit Gram-positive bacteria whilst glycine is added to suppress Gram-negative bacteria. GVPC medium can be used for water samples with a high concentration of background flora.
Prepare and sterilise the basal medium, including the glycine, as for BCYE agar. Add to the cooled medium (50°C) fresh filter-sterilised solutions of L-cysteine (0.4 g/10 mL), iron(III) pyrophosphate (0.25 g/10 mL), polymyxin B sulfate (80.000 i.u./10 mL), vancomycin hydrochloride (0.001 g/10 mL) and cycloheximide (0.08 g/10 mL).