This monograph has been reviewed by members of the IUMS–ICFMH Working Party on Culture Media and given ‘Proposed’ status.

This blood-free medium was developed for the selective isolation and quantification of arcobacters from food and environmental samples. Amphotericin B, cefoperazone, 5-fluorouracil, novobiocin and trimethoprim are incorporated as selective agents. For the isolation of low numbers of arcobacters from food and environmental samples, prior enrichment in Arcobacter selective isolation broth (Houf et al., 2001) should be performed.

Suspend the above ingredients, except those marked *, in the water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 min. Cool to 50°C. Suspend the required amount of amphotericin B, cefoperazone, 5-fluorouracil and novobiocin in 25 mL sterile distilled water. Suspend the required amount of trimethoprim in 25 mL sterile distilled water and add drops of lactic acid until trimethoprim is completely dissolved. Add aseptically both 25 mL solutions to the basal medium. Adjust the pH with NaOH.