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This monograph has been assessed by members of the IUMS–ICFMH Working Party on Culture Media and given ‘Approved’ status.

MRS agar was developed by de Man, Rogosa and Sharpe (1960) primarily for the cultivation of lactobacilli from various sources, with the intention of producing a defined medium as a substitute for tomato juice agar. It may be used for the cultivation of the whole group of lactic acid bacteria. The medium shows good productivity for nearly all lactic acid bacteria, but the original version is not selective. It may be made selective for lactic acid bacteria by lowering the pH to 5.7 and the addition of 0.14% sorbic acid (see MRS-S agar). Some strains from dairy sources may show reduced growth rates (see Briggs agar).

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