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This monograph has been reviewed by members of the IUMS–ICFMH Working Party on Culture Media and given ‘Proposed’ status.

This medium was originally developed by Kobayashi et al. (1963) for the isolation of Vibrio parahaemolyticus, but is also suitable for the isolation of Vibrio cholerae and most other pathogenic Vibrio spp. from clinical specimens. The selective components of the medium are bile salts, thiosulfate, citrate and a relatively high pH of 8.6. Differentiation is based on sucrose fermentation, detected by the pH indicators, and H2S production from thiosulfate, detected by the production of black iron sulfide from ferric citrate. Vibrio species do not produce H2S but, depending on the species, may ferment sucrose. Variations in the performance of different commercial preparations of this formula were noted by Nicholls et al. (1976) and West et al. (1982), who also addressed problems relating to quality control of the medium.

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