Handbook of Culture Media for Food and Water Microbiology
Chapter X2: Xylose Lysine Tergitol 4 (XLT4) agar
Published:06 Dec 2011
Special Collection: 2011 ebook collection , 2011 ebook collection , 2011-2015 food science subject collection
2011. "Xylose Lysine Tergitol 4 (XLT4) agar", Handbook of Culture Media for Food and Water Microbiology, Janet E L Corry, Gordon D W Curtis, R M Baird
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This monograph has been reviewed by members of the IUMS–ICFMH Working Party on Culture Media and given ‘Proposed’ status.
This medium was developed by Miller et al. (1991) as a modification of xylose lysine deoxycholate agar for the isolation of non-typhi Salmonella spp. The selectivity relies on the use of the detergent Niaproof 4 (7-ethyl-2-methylundecan-4-yl sulfate, sodium salt), formerly Tergitol 4. Its diagnostic properties are based on the use of three indicator systems: xylose, lactose and sucrose combined with phenol red; lysine hydrochloride and also phenol red; sodium thiosulfate and iron. The low concentration of proteose peptone no. 3 incorporated in the medium produces blacker salmonella colonies in shorter incubation times (Miller et al., 1995). XLT4 inhibits many Gram-negative bacteria and provides good colonial differentiation between Salmonella and Citrobacter spp.