Isoflavones: Chemistry, Analysis, Function and Effects
Isoflavones remain the subject of many scientific studies most of which reveal them to have some health benefits. Coverage within this book begins with an overview of phytoestrogens in health and plants with specific reference to isoflavones, how isoflavones are found in the diet and novel compounds in nuts. Expert accounts of the chemical and biochemical research on this topic are provided followed by analytical and bioanalytical assessments. Rounding up the book are the chapters on function and effects of isoflavones which provide details on isoflavones in beverages, soy and soy products and other food delivery systems and how their function effects the thyroid, menopause, prostate, breast, bone and cardiovascular disease to name but a few. Delivering high quality information, this extensive and detailed book provides a fascinating insight into this area of health and nutritional science. It will bridge scientific disciplines so that the information is more meaningful and applicable to health in general. Part of a series of books, it is specifically designed for chemists, analytical scientists, forensic scientists, food scientists, dieticians and health care workers, nutritionists, toxicologists and research academics. Due to its interdisciplinary nature it could also be suitable for lecturers and teachers in food and nutritional sciences and as a college or university library reference guide.
K. Nara, D. Liu, P. Kachlicki, R. C. Alves, R. Baber, F. Bucar, ... B. Stephens, Isoflavones: Chemistry, Analysis, Function and Effects, The Royal Society of Chemistry, 2012.
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CHAPTER 1: Phytoestrogens in Health: The Role of Isoflavonesp3-13ByRodney J. BaberRodney J. BaberMedical Suites, Level 3North Shore Private Hospital, Westbourne St.St Leonards, NSW 2065Australiarbaber@med.usyd.edu.auSearch for other works by this author on:
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CHAPTER 2: Phytoestrogens in Plants: With Special Reference to Isoflavonesp14-27ByFranz BucarFranz BucarInstitute of Pharmaceutical SciencesDepartment of PharmacognosyUniversity of Graz, Universitätsplatz 4, A-8010 GrazAustriafranz.bucar@uni-graz.atSearch for other works by this author on:
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CHAPTER 3: Isoflavones in Foods and Ingestion in the Dietp28-45ByBing Huei ChenBing Huei ChenSearch for other works by this author on:
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CHAPTER 4: The Chemistry/Biochemistry of the Bioconversion of Isoflavones in Food Preparationp49-60ByKashif Ghafoor,;Kashif Ghafoor,Department of Food and Nutrition Sciences, College of Food and Agricultural SciencesKing Saud UniversityP.O. Box 2460, Riyadh 11451Saudi ArabiaSearch for other works by this author on:Fahad Y. Al-Juhaimi;Fahad Y. Al-JuhaimiDepartment of Food and Nutrition Sciences, College of Food and Agricultural SciencesKing Saud UniversityP.O. Box 2460, Riyadh 11451Saudi ArabiaSearch for other works by this author on:Jiyong ParkJiyong ParkBiomaterials Process Engineering Laboratory, Department of BiotechnologyYonsei University134 Sinchon Dong, Seodaemun-gu, Seoul 120-749Republic of Koreafoodpro@yonsei.ac.krSearch for other works by this author on:
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CHAPTER 5: Chemistry and Synthesis of Daidzein and its Methylated Derivatives: Formononetin, Isoformononetin, and Dimethyldaidzeinp61-82ByVincent M. Carroll;Vincent M. CarrollDepartment of Chemistry, Niagara University206 DePaul HallNiagara UniversityNY, USAronny.priefer@wne.eduSearch for other works by this author on:Jeffrey D. St. Denis;Jeffrey D. St. DenisDepartment of Chemistry, Niagara University206 DePaul HallNiagara UniversityNY, USAronny.priefer@wne.eduSearch for other works by this author on:Kyle F. Biegasiewicz;Kyle F. BiegasiewiczDepartment of Chemistry, Niagara University206 DePaul HallNiagara UniversityNY, USAronny.priefer@wne.eduSearch for other works by this author on:Ronny PrieferRonny PrieferDepartment of Pharmaceutical & Administrative ScienceCollege of Pharmacy, Western New England UniversityCSP326-1215 Wilbraham Rd.Springfield, MA, USADepartment of Chemistry, Niagara University206 DePaul HallNiagara UniversityNY, USAronny.priefer@wne.eduSearch for other works by this author on:
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CHAPTER 6: Non-natural Isoflavonoidsp83-93ByNamita Bhan;Namita BhanDepartment of Chemical and Biological EngineeringRensselaer Polytechnic Institute110 8th Street, Troy, NY 12180USASearch for other works by this author on:Mattheos KoffasMattheos KoffasCenter for Biotechnology and Interdisciplinary StudiesRoom 4005D, Rensselaer Polytechnic Institute110 8th Street, Troy, NY 12180USAkoffam@rpi.eduSearch for other works by this author on:
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CHAPTER 7: The Structure of Isoflavones by 1D and 2D Homonuclear and Heteronuclear NMR Spectroscopyp94-114ByKristiina Wähälä,;Kristiina Wähälä,Department of Chemistry, Laboratory of Organic ChemistryUniversity of HelsinkiP. O. Box 55, FIN-00014Finlandkristiina.wahala@helsinki.fiSearch for other works by this author on:Somdatta Deb;Somdatta DebDepartment of Chemistry, Laboratory of Organic ChemistryUniversity of HelsinkiP. O. Box 55, FIN-00014Finlandkristiina.wahala@helsinki.fiSearch for other works by this author on:Tapio HaseTapio HaseDepartment of Chemistry, Laboratory of Organic ChemistryUniversity of HelsinkiP. O. Box 55, FIN-00014Finlandkristiina.wahala@helsinki.fiSearch for other works by this author on:
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CHAPTER 8: Biotransformation and Transfer of Genistein: a Comparison with Xenoestrogens and a Focus on the Human Placentap115-130ByHsiu-Wen Chan;Hsiu-Wen ChanUniversity of Queensland Centre for Clinical ResearchBuilding 71/918, Royal Brisbane and Women's Hospital Campus, Herston, QLD 4029Australiamurray.mitchell@uq.edu.auSearch for other works by this author on:Greg E. Rice;Greg E. RiceUniversity of Queensland Centre for Clinical ResearchBuilding 71/918, Royal Brisbane and Women's Hospital Campus, Herston, QLD 4029Australiamurray.mitchell@uq.edu.auSearch for other works by this author on:Murray D. MitchellMurray D. MitchellUniversity of Queensland Centre for Clinical ResearchBuilding 71/918, Royal Brisbane and Women's Hospital Campus, Herston, QLD 4029Australiamurray.mitchell@uq.edu.auSearch for other works by this author on:
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CHAPTER 9: The Biological Effects of Genistein and its Intracellular Metabolite, 5,7,3′,4′-Tetrahydroxyisoflavonep131-147ByGiulia Corona*;Giulia Corona*Molecular Nutrition GroupFood and Nutritional Sciences Department, School of Chemistry, Food and PharmacyUniversity of Reading, Reading RG6 6APUKg.corona@reading.ac.ukSearch for other works by this author on:Jeremy P. E. SpencerJeremy P. E. SpencerMolecular Nutrition GroupFood and Nutritional Sciences Department, School of Chemistry, Food and PharmacyUniversity of Reading, Reading RG6 6APUKg.corona@reading.ac.ukSearch for other works by this author on:
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CHAPTER 10: Genistein Chemistry and Biochemistryp148-156ByFrancesco Squadrito;Francesco SquadritoDepartment of Clinical and Experimental Medicine and PharmacologyUniversity of MessinaMessinaItalyFrancesco.Squadrito@unime.itSearch for other works by this author on:Alessandra BittoAlessandra BittoDepartment of Clinical and Experimental Medicine and PharmacologyUniversity of MessinaMessinaItalyFrancesco.Squadrito@unime.itSearch for other works by this author on:
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CHAPTER 11: Isoflavones and Human Estrogen Receptor: When Plants Synthesize Mammalian Hormone Mimeticsp157-176ByPatricia de Cremoux;Patricia de CremouxMolecular Oncology Unit, Department of Biochemistry, Saint-Louis HospitalUniversity Paris-Diderot1 avenue Claude Vellefaux, 75010 ParisFranceSearch for other works by this author on:Yves JacquotYves JacquotLaboratoire des BioMolécules – CNRS / UMR 7203, Department of Chemistry, Ecole Normale Supérieure (ENS)University Pierre & Marie Curie24 rue Lhomond, 75231 Paris Cedex 05Franceyves.jacquot@upmc.frSearch for other works by this author on:
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CHAPTER 12: Continuous Microwave-assisted Isoflavone Extractionp179-195ByDorin Boldor;Dorin BoldorDepartment of Biological and Agricultural Engineering, Louisiana State University Agricultural Center and College of AgricultureLouisiana State University A&M College149 E. B. Doran Bldg, LSU AgCenter, Baton Rouge, LA 70803USAdboldor@agcenter.lsu.eduSearch for other works by this author on:Cristina Mirela SabliovCristina Mirela SabliovDepartment of Biological and Agricultural Engineering, Louisiana State University Agricultural Center and College of AgricultureLouisiana State University A&M College149 E. B. Doran Bldg, LSU AgCenter, Baton Rouge, LA 70803USAdboldor@agcenter.lsu.eduSearch for other works by this author on:
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CHAPTER 13: Isoflavones: High-performance Liquid Chromatographic Analysis of Glucuronic Acid- and Sulfuric Acid-conjugated Metabolites of Daidzein and Genistein in Human Plasma and Urinep196-217ByKazuo Ishii;Kazuo IshiiDepartment of Clinical PharmacologySchool of Health SciencesKyorin University, 476 Miyashita, Hachioji, Tokyo 192-8508JapanSearch for other works by this author on:Kaori Hosoda;Kaori HosodaDepartment of Clinical PharmacologySchool of Health SciencesKyorin University, 476 Miyashita, Hachioji, Tokyo 192-8508JapanSearch for other works by this author on:Takashi FurutaTakashi FurutaDepartment of Medicinal Chemistry and Clinical PharmacySchool of PharmacyTokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392Japanishiikaz@ks.kyorin-u.ac.jpSearch for other works by this author on:
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CHAPTER 14: High-throughput Quantification of Pharmacologically Active Isoflavones using LC-UV/PDA and LC-MS/MSp218-243ByWahajuddin;WahajuddinPharmacokinetics and Metabolism DivisionCSIR – Central Drug Research InstitutePost Box No. 173, Chhattar Manzil Palace, Lucknow − 226001 (U. P.)IndiaSearch for other works by this author on:Sumit AroraSumit AroraDepartment of PharmaceuticsNational Institute of Pharmaceutical Education and ResearchRae Bareli–229001 (U. P.)Indiawahajuddin@cdri.res.inSearch for other works by this author on:
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CHAPTER 15: Methods for Isoflavones: A Focus on Beverage Analysisp244-262ByRita C. Alves;Rita C. AlvesREQUIMTE, Instituto Superior de Engenharia do Porto, Instituto Politécnico do PortoRua Dr. António Bernardino de Almeida, n°431, 4200-072 PortoPortugalREQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do PortoRua de Jorge Viterbo Ferreira n.° 228, 4050-313 PortoPortugalrita.c.alves@gmail.comSearch for other works by this author on:M. Beatriz P. P. OliveiraM. Beatriz P. P. OliveiraREQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do PortoRua de Jorge Viterbo Ferreira n.° 228, 4050-313 PortoPortugalrita.c.alves@gmail.comSearch for other works by this author on:
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CHAPTER 16: The Determination of Isoflavones in Supplemented Foods: An Overviewp263-279ByAlberto Zafra-Gómez;Alberto Zafra-GómezDepartment of Analytical ChemistryUniversity of GranadaAv. Fuentenueva S/N, GranadaSpain, E-18071azafra@ugr.esSearch for other works by this author on:Sonia Capel-Cuevas;Sonia Capel-CuevasDepartment of Analytical ChemistryUniversity of GranadaAv. Fuentenueva S/N, GranadaSpain, E-18071azafra@ugr.esSearch for other works by this author on:Noemí I. Dorival-GarcíaNoemí I. Dorival-GarcíaDepartment of Analytical ChemistryUniversity of GranadaAv. Fuentenueva S/N, GranadaSpain, E-18071azafra@ugr.esSearch for other works by this author on:
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CHAPTER 17: Isoflavones: LC-MS/MS Profiling of Isoflavone Glycosides and Other Conjugatesp280-293ByPiotr Kachlicki;Piotr KachlickiLaboratory of MetabolomicsInstitute of Plant Genetics, Polish Academy of SciencesStrzeszyńska 34, 60-479 PoznańPolandpkac@igr.poznan.plSearch for other works by this author on:Maciej StobieckiMaciej StobieckiLaboratory of Metabolomics and ProteomicsInstitute of Bioorganic Chemistry, Polish Academy of SciencesNoskowskiego 12/14, 61-704 PoznańPolandSearch for other works by this author on:
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CHAPTER 18: Puerariae radix Isoflavonesp294-315ByLei Wan;Lei WanSchool of Chinese MedicineChina Medical UniversityNo. 91 Hsueh-Shih Road, Taichung, Taiwan, and Department of Health and Nutrition Biotechnology, College of Health Science, No. 500, Lioufeng Road, Wufeng, TaichungTaiwanSearch for other works by this author on:Chia-Hung LinChia-Hung LinInstitute of Molecular MedicineNational Tsing Hua University101, Section 2, Kuang Fu Road, HsinchuTaiwanleiwan@mail.cmu.edu.twSearch for other works by this author on:
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CHAPTER 19: Pattern Profiling and Quantitative Determination of Isoflavones in Herbal Chemotypes using Liquid Chromatography Tandem Mass Spectrometryp316-332ByLakshmi Manickavasagam;Lakshmi ManickavasagamDivision of Pharmacokinetics and MetabolismCentral Drug Research InstitutePO Box-173, Lucknow-226001Indiagk_jain@cdri.res.inSearch for other works by this author on:Smriti Mishra;Smriti MishraDivision of Pharmacokinetics and MetabolismCentral Drug Research InstitutePO Box-173, Lucknow-226001Indiagk_jain@cdri.res.inSearch for other works by this author on:Girish Kumar JainGirish Kumar JainDivision of Pharmacokinetics and MetabolismCentral Drug Research InstitutePO Box-173, Lucknow-226001Indiagk_jain@cdri.res.inSearch for other works by this author on:
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CHAPTER 20: Analysis of Novel Isoflavone Digycoside in Nutsp333-346ByKazuhiro NaraKazuhiro NaraDepartment of Life DesignFaculty of Home EconomicsTokyo Kasei-Gakuin University, Machida, Tokyo 194-0292Japank_nara@kasei-gakuin.ac.jpSearch for other works by this author on:
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CHAPTER 21: Isoflavone Ingestion by Multiethnic Populations: Implications for Healthp349-364ByBing Huei ChenBing Huei ChenSearch for other works by this author on:
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CHAPTER 22: Isoflavones in Beveragesp365-380ByRita C. Alves;Rita C. AlvesREQUIMTEInstituto Superior de Engenharia do PortoInstituto Politécnico do Porto, Rua Dr. António Bernardino de Almeidan° 431, 4200-072 Porto, PortugalREQUIMTE, Departamento de Ciências Químicas, Faculdade de FarmáciaUniversidade do Porto, Rua de Jorge Viterbo Ferreira n° 2284050-313 PortoPortugalrita.c.alves@gmail.comSearch for other works by this author on:M. Beatriz P. P. OliveiraM. Beatriz P. P. OliveiraREQUIMTE, Departamento de Ciências Químicas, Faculdade de FarmáciaUniversidade do Porto, Rua de Jorge Viterbo Ferreira n° 2284050-313 PortoPortugalrita.c.alves@gmail.comSearch for other works by this author on:
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CHAPTER 23: Use of Isoflavones in Inherited Metabolic Diseases: A Focus on Mucopolysaccharidosesp381-398ByDaniel Scherman,*;Daniel Scherman,*CNRS UMR 8151; INSERM U1022, Ecole Nationale Supérieure de Chimie de Paris, Chimie ParisTech, Université Paris Descartes, Sorbonne Paris Cité, Faculté de PharmacieChemical and Genetic Pharmacology Laboratory4 avenue de l’Observatoire 75006, ParisFranceDaniel.Scherman@parisdescartes.frSearch for other works by this author on:
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CHAPTER 24: Optimizing Isoflavone-rich Food Delivery Systems for Human Clinical Trialsp399-422ByJennifer Ahn-Jarvis;Jennifer Ahn-JarvisDepartment of Food Science and TechnologyCollege of FoodAgricultural, and Environmental Sciences, The Ohio State University, 2015 Fyffe Court, Columbus, Ohio, USAvodovotz.1@osu.eduSearch for other works by this author on:Steven Schwartz;Steven SchwartzDepartment of Food Science and TechnologyCollege of FoodAgricultural, and Environmental Sciences, The Ohio State University, 2015 Fyffe Court, Columbus, Ohio, USAvodovotz.1@osu.eduSearch for other works by this author on:Yael VodovotzYael VodovotzDepartment of Food Science and TechnologyCollege of FoodAgricultural, and Environmental Sciences, The Ohio State University, 2015 Fyffe Court, Columbus, Ohio, USAvodovotz.1@osu.eduSearch for other works by this author on:
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CHAPTER 25: Isoflavones and Thyroid Function: An Overviewp423-437ByFrancesco Squadrito;Francesco SquadritoDepartment of Clinical and Experimental Medicine and PharmacologyUniversity of MessinaMessinaItalyFrancesco.Squadrito@unime.itSearch for other works by this author on:Alessandra BittoAlessandra BittoDepartment of Clinical and Experimental Medicine and PharmacologyUniversity of MessinaMessinaItalyFrancesco.Squadrito@unime.itSearch for other works by this author on:
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CHAPTER 26: Isoflavones against Gastric Cancer: Function and Effectsp438-450BySue K. Park;Sue K. ParkDepartment of Preventive MedicineSeoul National University College of MedicineSeoulKoreaDepartment of Biomedical ScienceSeoul National University Graduate SchoolSeoulKoreaCancer Research InstituteSeoul National University, SeoulKoreaSearch for other works by this author on:Kwang-Pil KoKwang-Pil KoSearch for other works by this author on:
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CHAPTER 27: Dietary Isoflavones and Learning and Memoryp451-464ByCraige C. WrennCraige C. WrennDepartment of Pharmaceutical, Biomedical, and Administrative SciencesCollege of Pharmacy and Health Sciences, Drake UniversityDes Moines, IA 50311USAcraige.wrenn@drake.eduSearch for other works by this author on:
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CHAPTER 28: Glycitein in Healthp465-479ByBrian R. Stephens;Brian R. StephensDepartment of Human NutritionOhio State University1787 Neil Ave., Columbus, Ohio 43210USAbomser.1@osu.eduSearch for other works by this author on:Joshua A. BomserJoshua A. BomserDepartment of Human NutritionOhio State University1787 Neil Ave., Columbus, Ohio 43210USAbomser.1@osu.eduSearch for other works by this author on:
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CHAPTER 29: Isoflavones and Prenatal Exposure to Equolp480-499ByEdwin D. LephartEdwin D. LephartDepartment of Physiology and Developmental Biology and The Neuroscience CenterCollege of Life Sciences, 785 WIDB, Brigham Young University, Provo, UtahUSAEdwin_Lephart@byu.eduSearch for other works by this author on:
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CHAPTER 30: Genistein: GABAA and NMDA Receptorsp500-517ByRenqi Huang;Renqi HuangDepartment of Pharmacology and NeuroscienceUniversity of North Texas Health Science CenterFort Worth, TXUSARen-qi.huang@unthsc.eduSearch for other works by this author on:Glenn H. DillonGlenn H. DillonDepartment of Pharmacology and NeuroscienceUniversity of North Texas Health Science CenterFort Worth, TXUSARen-qi.huang@unthsc.eduSearch for other works by this author on:
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CHAPTER 31: Estrogenic Activity and Molecular Mechanisms of Coumestrol-induced Biological Effectsp518-528ByKenneth Ndebele;Kenneth NdebeleDepartment of Biology, College of Science, Engineering and TechnologyJackson State University1400 J. R. Lynch Street, Jackson, MS 39217USApaul.b.tchounwou@jsums.eduSearch for other works by this author on:Barbara Graham;Barbara GrahamDepartment of Biology, College of Science, Engineering and TechnologyJackson State University1400 J. R. Lynch Street, Jackson, MS 39217USApaul.b.tchounwou@jsums.eduSearch for other works by this author on:Paul Tchounwou*Paul Tchounwou*Department of Biology, College of Science, Engineering and TechnologyJackson State University1400 J. R. Lynch Street, Jackson, MS 39217USApaul.b.tchounwou@jsums.eduSearch for other works by this author on:
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CHAPTER 32: Genistein and Insulin Secretory Functionp529-540ByDongmin LiuDongmin LiuDepartment of Human NutritionFoods and ExerciseCollege of Agriculture and Life Sciences, Virginia Tech, Blacksburg, Virginia 24061USAdoliu@vt.eduSearch for other works by this author on:
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CHAPTER 33: Prevention and Management of Obesity by Isoflavonesp541-561ByBarbara B. Doonan;Barbara B. DoonanDepartment of Biochemistry and Molecular BiologyNew York Medical CollegeValhalla, NY 10595USAJoseph_Wu@nymc.eduSearch for other works by this author on:Erxi Wu;Erxi WuDepartment of Pharmaceutical SciencesNorth Dakota State UniversityFargo, ND 58105USASearch for other works by this author on:Joseph M. WuJoseph M. WuDepartment of Biochemistry and Molecular BiologyNew York Medical CollegeValhalla, NY 10595USAJoseph_Wu@nymc.eduSearch for other works by this author on:
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CHAPTER 34: Soy Isoflavones and Testicular Functionp562-579ByBenson T. AkingbemiBenson T. AkingbemiSearch for other works by this author on:
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CHAPTER 35: Equol and Cell Proliferationp580-596ByZhong Li;Zhong LiDepartment of Nutrition and Food HygieneSchool of Public Health, Nanjing Medical University140 Hanzhong Rd, Nanjing 210029People's Republic of Chinauiuclz@126.comSearch for other works by this author on:Caiyun Zhong;Caiyun ZhongDepartment of Nutrition and Food HygieneSchool of Public Health, Nanjing Medical University140 Hanzhong Rd, Nanjing 210029People's Republic of Chinauiuclz@126.comSearch for other works by this author on:Chunyan HuChunyan HuDepartment of Nutrition and Food HygieneSchool of Public Health, Nanjing Medical University140 Hanzhong Rd, Nanjing 210029People's Republic of Chinauiuclz@126.comSearch for other works by this author on:
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CHAPTER 36: Bone, Genistein, Daidzein and Equolp597-610ByMarina Komrakova;Marina KomrakovaDepartment of Trauma Surgery and Reconstructive SurgeryUniversity Medical Center GoettingenRobert-Koch St. 40, GoettingenGermanykomrakova@yahoo.comSearch for other works by this author on:Ewa Klara Stuermer;Ewa Klara StuermerDepartment of Trauma Surgery and Reconstructive SurgeryUniversity Medical Center GoettingenRobert-Koch St. 40, GoettingenGermanykomrakova@yahoo.comSearch for other works by this author on:Klaus Michael Stuermer;Klaus Michael StuermerDepartment of Trauma Surgery and Reconstructive SurgeryUniversity Medical Center GoettingenRobert-Koch St. 40, GoettingenGermanykomrakova@yahoo.comSearch for other works by this author on:Stephan SehmischStephan SehmischDepartment of Trauma Surgery and Reconstructive SurgeryUniversity Medical Center GoettingenRobert-Koch St. 40, GoettingenGermanykomrakova@yahoo.comSearch for other works by this author on:
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CHAPTER 37: Isoflavones and Inflammation in Adipose Tissue and Implications for Healthp611-626ByMaria Teresa Blay;Maria Teresa BlayDepartament de Bioquímica i BiotecnologiaGrup de Recerca en NutrigenòmicaUniversitat Rovira i Virgili, TarragonaSpainmteresa.blay@urv.catSearch for other works by this author on:Montserrat Pinent;Montserrat PinentDepartament de Bioquímica i BiotecnologiaGrup de Recerca en NutrigenòmicaUniversitat Rovira i Virgili, TarragonaSpainmteresa.blay@urv.catSearch for other works by this author on:Anna ArdévolAnna ArdévolDepartament de Bioquímica i BiotecnologiaGrup de Recerca en NutrigenòmicaUniversitat Rovira i Virgili, TarragonaSpainmteresa.blay@urv.catSearch for other works by this author on:
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CHAPTER 38: Isoflavones for Menopausal Vasomotor Syndromep627-645ByRafael Bolaños Diaz, MD, MSc;Rafael Bolaños Diaz, MD, MScCalle 9n° 241, Dpt. 101-A, Urb. Monterrico Norte, San Borja, Lima 41Lima, Perurbolanosd@correo.unmsm.edu.pe; rbolanosd@yahoo.esSearch for other works by this author on:Juan Carlos Zavala Gonzales, MD, MScJuan Carlos Zavala Gonzales, MD, MScAv. Angamos-EsteCdra. 26, F 47, San Borja (Lima 41), LimaPerúSearch for other works by this author on:
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