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As a rising star of two-dimensional (2D) materials, 2D organic materials have inspired massive interest due to their remarkable merits such as a large materials library, intrinsic flexibility, diverse synthesis techniques, etc., which provide them with great prospects for flexible optoelectronics applications. Moreover, highly ordered 2D organic materials exhibit ultrathin features, low symmetry and unique anisotropy, and all these open a new avenue to achieve high-performance 2D organic materials devices and benefit their integration into optoelectronics. Herein, we first review several important growth techniques of 2D organic materials. Second, we summarize the recent progress in anisotropy characterizations of 2D organic materials and their applications in the optoelectronics field. Finally, we presented an outlook of anisotropic 2D organic materials in terms of challenges and opportunities. We believe this chapter will be an important reference for designing and developing novel 2D organic materials and integrating them into next-generation optoelectronics, provoking more researchers to come to this field.

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