Chapter 11: Describing Dynamic Chaperone–Client Complexes by Solution NMR Spectroscopy
Published:17 Aug 2022
S. Hiller and B. M. Burmann, in NMR Spectroscopy for Probing Functional Dynamics at Biological Interfaces, ed. A. Bhunia, H. S. Atreya, and N. Sinha, The Royal Society of Chemistry, 2022, ch. 11, pp. 277-302.
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Chaperone–client interactions are highly dynamic in their nature and result in transient, multi-conformational complexes that are challenging to characterize at the atomic level. Solution NMR spectroscopy has the power to resolve structure and dynamics even for large molecular systems in aqueous solution and is therefore a method of choice for chaperone–client systems. Here, we categorize the NMR techniques suitable for the characterization of dynamic complexes, assess their particular usefulness for chaperone–client complexes and describe how the resulting data can be fruitfully interpreted toward structural models.