Chapter 17: Membranes, Minerals and Magnets: Application of NMR Spectroscopy to Biological Interfaces
Published:17 Aug 2022
J. E. Kent, K. Shin, and F. M. Marassi, in NMR Spectroscopy for Probing Functional Dynamics at Biological Interfaces, ed. A. Bhunia, H. S. Atreya, and N. Sinha, The Royal Society of Chemistry, 2022, ch. 17, pp. 496-529.
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Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for studying the structures and interactions of macromolecules and their assemblies with atomic resolution. It is the premier technology for characterizing the complex biology occurring at interfacial regions, a challenging task where samples are often not amenable for X-ray crystallography or electron microscopy (EM) methods. This chapter describes examples of the successful application of NMR spectroscopy to complex biological systems ranging from membrane-embedded proteins to mineral-associated proteins and describes advancements in the field to obtain native and near-native systems.