Chapter 5: Diversity-oriented Fluorescence Library Approach: Accelerating Probe Development for Biological and Environmental Applications
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Published:14 Apr 2023
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Special Collection: 2023 ebook collection
A. Samanta, S. Munan, A. Jana, and Y. T. Chang, in Fluorescent Chemosensors, ed. L. Wu, A. C. Sedgwick, X. He, and T. D. James, The Royal Society of Chemistry, 2023, ch. 5, pp. 67-105.
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In chemical biology the concept of chemosensors and chemical probes implicates the transduction of a chemical signal upon binding with the chemical substance. There are enormous challenges for developing superior chemical probes using the target-oriented synthesis approach with limited knowledge of the recognition site. The emerging field of the diversity-oriented fluorescent library approach (DOFLA) enables a conceptually novel and new strategy for designing chemosensors/probes. Vast chemical diversity in optically active fluorophores provides limitless opportunity for probe development, likewise drug discovery from diversity-oriented synthesis approaches. Synergistic effects of diverse chemical structures, high-throughput screening methodology, and improved microscopic automation have led to several chemical probes in the last two decades. This chapter summarizes the recent developments of chemical probes/sensors and chemosensors using DOFLA and their biological and environmental applications. The chapter consists of six sections, including the concept of the diversity-oriented fluorescent library, mechanisms of signal transduction, screening strategies, and reviews of recent examples from in vitro spectra-based screening, cell-based screening, and in vivo whole organelle-based screening.