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The fabrication, construction, and composition of multicomponent hydrogels are complex. In recent years, more novel applications of multicomponent hydrogels, such as for three-dimensional printing or in cultures to establish artificial tissues or organs, have been reported. The basic material properties of multicomponent hydrogels should be analyzed to develop new applications. Thus, in this chapter, three different types of analyses are reviewed and summarized, namely surface, physicochemical, and biological characterization, to cover this growing multidisciplinary field of research. Furthermore, the mechanism and features of each technique are discussed in this chapter.

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