Detection and Analysis of Microorganisms by Mass Spectrometry
In the human body, there are millions of living microorganisms involved in protecting the body from invaders, helping digestion and regulating moods, but there are also harmful pathogens that cause infectious diseases. For instance, the coronavirus (COVID-19) has caused considerable loss of life since its outbreak. Comprehensive analysis and characterization of microbes is of significant importance to understand the function and role of microorganisms, and rapid detection and identification of unknown pathogens are essential in early diagnosis, treatment monitoring and personalized medicine.
Mass spectrometry is a technique to ionize molecules and detect the mass-to-charge ratio of the generated ions. The technique is widely used in hospitals for pathogenic bacteria identification, as well as in environmental science and food science for biosafety control. This book summarizes the most recent development of mass spectrometry techniques in microbial analysis, including mass spectrometry-based microbial identification, bacterial antimicrobial resistance study, data mining algorithm development, omics for microbial research, applications in clinical diagnosis, environmental science and food science, and more. It will guide researchers in the field, and those who are about to enter the field, in the most appropriate methods to characterize microbes and enable their detection.
Detection and Analysis of Microorganisms by Mass Spectrometry, Royal Society of Chemistry, 2023.
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Table of contents
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Introduction of Mass Spectrometry-based Microorganism Detectionp1-26ByJia Yi;Jia YiChemistry Department, Fudan University, Shanghai, 200000, ChinaSearch for other works by this author on:Dongxue Zhang;Dongxue ZhangChemistry Department, Fudan University, Shanghai, 200000, ChinaSearch for other works by this author on:Liang QiaoLiang QiaoChemistry Department, Fudan University, Shanghai, 200000, ChinaSearch for other works by this author on:
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Chapter 2: MALDI-TOF Mass Fingerprinting for Rapid Identification of Bacteria: Experimental Proceduresp27-44ByYingdi Zhu;Yingdi ZhuaDepartment of Chemistry and Chemical Engineering, Laboratory of Analytical and Physical Electrochemistry, École Polytechnique Fédérale de Lausanne, Station 6, 1015 Lausanne, SwitzerlandbInstitute of Basic Medicine and Cancer, Chinese Academy of Sciences, Fucheng Road 150, 310000 Hangzhou, ChinaSearch for other works by this author on:Hubert H. GiraultHubert H. GiraultaDepartment of Chemistry and Chemical Engineering, Laboratory of Analytical and Physical Electrochemistry, École Polytechnique Fédérale de Lausanne, Station 6, 1015 Lausanne, SwitzerlandSearch for other works by this author on:
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Chapter 3: MALDI-TOF Mass Fingerprinting for Rapid Identification of Bacteria: Data Analysis and Algorithm Developmentp45-75ByY. YangY. YangZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 311200, ChinaSearch for other works by this author on:
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Chapter 4: Ambient Ionisation Mass Spectrometry for Microbial Analysisp76-98ByN. StrittmatterN. StrittmatterDepartment of Biosciences, School of Natural Sciences, Technical University of Munich, 85748 Garching, GermanySearch for other works by this author on:
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Chapter 5: Nucleic Acid Amplification Coupled with Mass Spectrometry Detection for Microbial Identificationp99-124ByAizhong Hu;Aizhong HuaVacTx, 13 Melody Lane, Harriman, NY10926, USASearch for other works by this author on:Qingwei MaQingwei MacBioyong Co. Ltd, ChinaSearch for other works by this author on:
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Chapter 6: Proteomics Analysis for Identification and Antimicrobial Resistance Analysis of Bacteriap125-153ByDongxue ZhangDongxue ZhangInstitutes of Biomedical Sciences, Fudan University, Shanghai 200000, ChinaSearch for other works by this author on:
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Chapter 7: Metabolomics as a Tool for Phenotypic Characterization and Strain Improvementp154-172ByMarvin Nathanael Iman;Marvin Nathanael ImanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, JapanSearch for other works by this author on:Rifqi Ahmad Riyanto;Rifqi Ahmad RiyantoDepartment of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, JapanSearch for other works by this author on:Sastia Prama PutriSastia Prama PutriDepartment of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, JapanSearch for other works by this author on:
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Chapter 8: Integrated Analysis for Identification, Phenotyping, and Antimicrobial Susceptibility Testing (AST) of Bacteria Using Mass Spectrometry, Machine Learning, and Multi-omics Analysisp173-187ByRutan Zhang;Rutan ZhangaDepartment of Medicinal Chemistry, University of Washington, Seattle, WA, USASearch for other works by this author on:Brian J. Werth;Brian J. WerthbDepartment of Pharmacy, University of Washington, Seattle, WA, USASearch for other works by this author on:Libin XuLibin XuaDepartment of Medicinal Chemistry, University of Washington, Seattle, WA, USASearch for other works by this author on:
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Chapter 9: Rapid Detection and Evolution of SARS-CoV2 and Other Viruses with High Resolution Mass Spectrometryp188-214ByKevin M. DownardKevin M. DownardaInfectious Disease Responses Laboratory, Prince of Wales Clinical Research Sciences, Sydney, NSW, AustraliaSearch for other works by this author on:
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Chapter 10: Mass Spectrometry-based Proteomics of Eukaryotesp215-233ByJennifer Geddes-McAlister;Jennifer Geddes-McAlisteraDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, CanadabCanadian Proteomics and Artificial Intelligence Consortium, CanadaSearch for other works by this author on:Tia Rizakos;Tia RizakosaDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, CanadaSearch for other works by this author on:Benjamin MuseliusBenjamin MuseliusaDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, CanadaSearch for other works by this author on:
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Chapter 11: Mass Spectrometry Imaging in Microbiologyp234-262ByYuchen Zou;Yuchen ZouState Key Laboratory of Natural Medicines and School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, ChinaSearch for other works by this author on:Bin LiBin LiState Key Laboratory of Natural Medicines and School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, ChinaSearch for other works by this author on:
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Chapter 12: Identification of Microorganisms by Mass Spectrometry in Clinical Microbiology Laboratoriesp263-276ByQin Qin;Qin QinShanghai Changhai Hospital, Changhai Road 168, Shanghai 200433, ChinaSearch for other works by this author on:Yun Liu;Yun LiuShanghai Changhai Hospital, Changhai Road 168, Shanghai 200433, ChinaSearch for other works by this author on:Yuxiang Wan;Yuxiang WanShanghai Changhai Hospital, Changhai Road 168, Shanghai 200433, ChinaSearch for other works by this author on:Haifeng QinHaifeng QinShanghai Changhai Hospital, Changhai Road 168, Shanghai 200433, ChinaSearch for other works by this author on:
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Chapter 13: Mass Spectrometry-based Microbial Identification and Profiling for Environmental Sciencep277-302BySimon J. S. CameronSimon J. S. CameronSchool of Biological Sciences, Institute for Global Food Security, Queen’s University Belfast, UKSearch for other works by this author on:
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Chapter 14: Mass Spectrometry (MS)-based Bacterial Identification in the Food Industryp303-327ByHongyan BiHongyan BiCollege of Food Science and Engineering, Shanghai Ocean University, Hucheng Ring Road 999, Pudong New District, 201306 Shanghai, ChinaSearch for other works by this author on:
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