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Eukaryotes are diverse and expansive, encompassing many forms of natural life, including animals, plants, and fungi. To explore these complex biological systems and better understand mechanisms regulating health and disease, advanced technological approaches are needed. Mass spectrometry (MS)-based proteomics combined with sophisticated bioinformatics tools can provide new mechanistic insight into these biological systems and regulation of diverse processes. In this chapter, we provide an overview of common MS-based proteomics tools used for discovery-centric investigations and focus on proteomics platforms applied for disease diagnostic and prognostic purposes, emphasizing the relationship between the immune system of the host and fungal pathogens.

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