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New Developments in Mass Spectrometry
Detection and Analysis of Microorganisms by Mass Spectrometry
Edited by
Jia Yi
Jia Yi
Fudan University, China
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Volume
13
Hardback ISBN:
978-1-83916-763-8
PDF ISBN:
978-1-83767-033-8
EPUB ISBN:
978-1-83767-034-5
Special Collection:
2023 ebook collection
No. of Pages:
360
Publication date:
06 Oct 2023
Book Chapter
Chapter 2: MALDI-TOF Mass Fingerprinting for Rapid Identification of Bacteria: Experimental Procedures
By
Yingdi Zhu
;
Yingdi Zhu
aDepartment of Chemistry and Chemical Engineering, Laboratory of Analytical and Physical Electrochemistry, École Polytechnique Fédérale de Lausanne, Station 6, 1015 Lausanne, Switzerland
bInstitute of Basic Medicine and Cancer, Chinese Academy of Sciences, Fucheng Road 150, 310000 Hangzhou, China
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Hubert H. Girault
Hubert H. Girault
aDepartment of Chemistry and Chemical Engineering, Laboratory of Analytical and Physical Electrochemistry, École Polytechnique Fédérale de Lausanne, Station 6, 1015 Lausanne, Switzerland
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Published:06 Oct 2023
-
Special Collection: 2023 ebook collection
Page range:
27 - 44
Citation
Y. Zhu and H. H. Girault, in Detection and Analysis of Microorganisms by Mass Spectrometry, ed. L. Qiao and J. Yi, Royal Society of Chemistry, 2023, vol. 13, ch. 2, pp. 27-44.
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In this chapter, scientific and instrumental fundamentals related to the generation of bacterial MALDI-TOF mass fingerprints were briefly described. For bacterial identification by MALDI-TOF mass fingerprinting, experimental procedures for patient sampling, the culture and isolation of bacterial cells from patient samples, the preparation of bacterial cells and MALDI matrices, and the deposition of bacteria and matrices on MALDI target plates, as well as the setting of instrumental parameters, were given in detail.
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