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The increase in environmental pollution and its impact on human health calls for proper investigation of the levels of chemicals and biomolecules so that a pollution-free environment can be created which will provide a healthy lifestyle. This requirement for appropriate real-time analysis of analytes has led to the current domination of research interest in sensing applications. Graphene-based polymer nanocomposites (GPNCs) have emerged as prominent and effective chemical and biosensing tools for diverse types of analytes. Detection of heavy metal ions, gases, hydrogen peroxide (H2O2), nitrophenols, bisphenol A, hydrazine, amino acids, glucose, cholesterol and microbes with the help of GPNCs is discussed in this chapter. The proposed mechanisms for detection of individual analytes by GPNCs are also described in order to explain the sensing process.

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